Cucumber hybrid sv8592cs

ABSTRACT

The invention provides seeds and plants of cucumber hybrid SV8592CS. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of cucumber hybrid SV8592CS and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another plant, such as a cucumber plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of cucumber hybrid SV8592CS comprising introduced beneficial or desirable traits.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of cucumber hybrid SV8592CS.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety. Such desirable traits may include any trait deemedbeneficial or desirable by a grower or consumer, including greateryield, resistance to insects or disease, tolerance to environmentalstress, and nutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all genetic loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for manygenetic loci. Conversely, a cross of two plants each heterozygous at anumber of loci produces a population of hybrid plants that differgenetically and are not uniform. The resulting non-uniformity makesperformance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a cucumber plant of hybridSV8592CS. Also provided are cucumber plants having all the physiologicaland morphological characteristics of such a plant. Parts of thesecucumber plants are also provided, for example, including pollen, anovule, an embryo, a seed, a scion, a rootstock, a fruit, and a cell ofthe plant.

In another aspect of the invention, a plant of cucumber hybrid SV8592CScomprising an added heritable trait is provided. The heritable trait maycomprise a genetic locus that is, for example, a dominant or recessiveallele. In one embodiment of the invention, a plant of cucumber hybridSV8592CS is defined as comprising a single locus conversion. In specificembodiments of the invention, an added genetic locus confers one or moretraits such as, for example, herbicide tolerance, insect resistance,disease resistance, and modified carbohydrate metabolism. In furtherembodiments, the trait may be conferred by a naturally occurring geneintroduced into the genome of a line by backcrossing, a natural orinduced mutation, or a transgene introduced through genetictransformation techniques into the plant or a progenitor of any previousgeneration thereof. When introduced through transformation, a geneticlocus may comprise one or more genes integrated at a single chromosomallocation.

In some embodiments, a single locus conversion includes one or moresite-specific changes to the plant genome, such as, without limitation,one or more nucleotide modifications, deletions, or insertions. A singlelocus may comprise one or more genes or nucleotides integrated ormutated at a single chromosomal location. In one embodiment, a singlelocus conversion may be introduced by a genetic engineering technique,methods of which include, for example, genome editing with engineerednucleases (GEEN). Engineered nucleases include, but are not limited to,Cas endonucleases; zinc finger nucleases (ZFNs); transcriptionactivator-like effector nucleases (TALENs); engineered meganucleases,also known as homing endonucleases; and other endonucleases for DNA orRNA-guided genome editing that are well-known to the skilled artisan.

The invention also concerns the seed of cucumber hybrid SV8592CS. Theseed of the invention may be provided as an essentially homogeneouspopulation of seed of cucumber hybrid SV8592CS. Essentially homogeneouspopulations of seed are generally free from substantial numbers of otherseed. Therefore, seed of cucumber hybrid SV8592CS may be defined asforming at least about 97% of the total seed, including at least about98%, 99%, or more of the seed. The seed population may be separatelygrown to provide an essentially homogeneous population of cucumberplants designated SV8592CS.

In yet another aspect of the invention, a tissue culture of regenerablecells of a cucumber plant of hybrid SV8592CS is provided. The tissueculture will preferably be capable of regenerating cucumber plantscapable of expressing all of the physiological and morphologicalcharacteristics of the starting plant and of regenerating plants havingsubstantially the same genotype as the starting plant. Examples of someof the physiological and morphological characteristics of cucumberhybrid SV8592CS include those traits set forth in the tables herein. Theregenerable cells in such tissue cultures may be derived, for example,from embryos, meristems, cotyledons, pollen, leaves, anthers, roots,root tips, pistils, flowers, seed, and stalks. Still further, thepresent invention provides cucumber plants regenerated from a tissueculture of the invention, the plants having all the physiological andmorphological characteristics of cucumber hybrid SV8592CS.

In still yet another aspect of the invention, processes are provided forproducing cucumber seeds, plants, and fruit, which processes generallycomprise crossing a first parent cucumber plant with a second parentcucumber plant, wherein at least one of the first or second parentplants is a plant of cucumber hybrid SV8592CS. These processes may befurther exemplified as processes for preparing hybrid cucumber seed orplants, wherein a first cucumber plant is crossed with a second cucumberplant of a different, distinct genotype to provide a hybrid that has, asone of its parents, a plant of cucumber hybrid SV8592CS. In theseprocesses, crossing will result in the production of seed. The seedproduction occurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent cucumber plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent cucumber plants into plants that bear flowers. A thirdstep may comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent cucumber plants. Yet another step comprisesharvesting the seeds from at least one of the parent cucumber plants.The harvested seed can be grown to produce a cucumber plant or hybridcucumber plant.

The present invention also provides the cucumber seeds and plantsproduced by a process that comprises crossing a first parent cucumberplant with a second parent cucumber plant, wherein at least one of thefirst or second parent cucumber plants is a plant of cucumber hybridSV8592CS. In one embodiment of the invention, cucumber seed and plantsproduced by the process are first generation (F₁) hybrid cucumber seedand plants produced by crossing a plant in accordance with the inventionwith another, distinct plant. The present invention further contemplatesplant parts of such an F₁ hybrid cucumber plant, and methods of usethereof. Therefore, certain exemplary embodiments of the inventionprovide an F₁ hybrid cucumber plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from cucumber hybrid SV8592CS, the methodcomprising the steps of: (a) preparing a progeny plant derived fromcucumber hybrid SV8592CS, wherein said preparing comprises crossing aplant of cucumber hybrid SV8592CS with a second plant; and (b) crossingthe progeny plant with itself or a second plant to produce a seed of aprogeny plant of a subsequent generation. In further embodiments, themethod may additionally comprise: (c) growing a progeny plant of asubsequent generation from said seed of a progeny plant of a subsequentgeneration and crossing the progeny plant of a subsequent generationwith itself or a second plant; and repeating the steps for an additional3-10 generations to produce a plant derived from cucumber hybridSV8592CS. The plant derived from cucumber hybrid SV8592CS may be aninbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom cucumber hybrid SV8592CS is obtained which possesses some of thedesirable traits of the line/hybrid as well as potentially otherselected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of cucumberhybrid SV8592CS, wherein the plant has been cultivated to maturity, and(b) collecting at least one cucumber from the plant.

In still yet another aspect of the invention, the genetic complement ofcucumber hybrid SV8592CS. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a cucumberplant, or a cell or tissue of that plant. A genetic complement thusrepresents the genetic makeup of a cell, tissue or plant, and a hybridgenetic complement represents the genetic make-up of a hybrid cell,tissue or plant. The invention thus provides cucumber plant cells thathave a genetic complement in accordance with the cucumber plant cellsdisclosed herein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that cucumber hybrid SV8592CS could be identified byany of the many well-known techniques such as, for example, SimpleSequence Length Polymorphisms (SSLPs) (Williams et al., Nucleic AcidsRes., 1 8:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs),DNA Amplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by cucumber plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a cucumber plant of the invention with a haploid geneticcomplement of a second cucumber plant, preferably, another, distinctcucumber plant. In another aspect, the present invention provides acucumber plant regenerated from a tissue culture that comprises a hybridgenetic complement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have,” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes,” and“including,” are also open-ended. For example, any method that“comprises,” “has,” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has,” or “includes” oneor more traits is not limited to possessing only those one or moretraits and covers other unlisted traits.

Other objects, features, and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds, and derivatives of cucumber hybrid SV8592CS, cucumber lineASL-147-1002-GY, and cucumber line ASL-M3092029MO.

Hybrid SV8592CS, also known as SVR14784822, is a gynoecious, Americanslicing variety intended for open-field production. The hybrid producespredominantly female flowers and is blended with a monecious pollinatorfor optimum production. The hybrid develops a plant that produces fruitsthat are dark-green and blocky with blunt ends and an attractive shape.The variety comprises an indeterminate plant habit and a greaterresistance to downy mildew compared to publicly-available hybrids Cortezand Cobra. The variety may also have resistance to anthracnose, angularleaf spot, powdery mildew, scab, cucumber mosaic virus, and zucchiniyellow mosaic virus.

A. Origin and Breeding History of Cucumber Hybrid SV8592CS

The parents of cucumber hybrid SV8592CS are cucumber lineASL-147-1002-GY and cucumber line ASL-M3092029MO. The parent lines areuniform and stable, as is a hybrid produced therefrom. A smallpercentage of variants can occur within commercially acceptable limitsfor almost any characteristic during the course of repeatedmultiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Cucumber HybridSV8592CS

In accordance with one aspect of the present invention, there areprovided plants having the physiological and morphologicalcharacteristics of cucumber hybrid SV8592CS. Descriptions of thephysiological and morphological characteristics of such plants arepresented in the table that follows.

TABLE 1 Physiological and Morphological Characteristics of CucumberHybrid SV8592CS SV8592CS CHARACTERISTIC (SVR14784822) DASHER II Typepredominant culture outdoor outdoor Maturity days from seeding to market62   65   maturity Plant habit vine vine cotyledon: bitterness absentpresent growth type indeterminate indeterminate time of development offemale early early flowers (80% of plants with at least one femaleflower) sex monoecious monoecious sex expression monoecious monoeciousnumber of female flowers per node mostly 1 mostly 1 flower color yellowyellow flower color (RHS color chart value)  13B  9A Main Stem main stemlength (cm) 104.0  111.8  number of nodes from cotyledon  3.3  3.2leaves to node bearing the first pistillate flower internode length (cm) 5.5  5.7 stem form grooved, ridged grooved, ridged plant: total lengthof first 15 medium medium internodes leaf blade: attitude horizontalhorizontal Leaf mature blade of third leaf: leaf 122.3  121.1  length(mm) mature blade of third leaf: leaf width 154.6  145.4  (mm) matureblade of third leaf: petiole 10.7 14.3 length (cm) length long longratio length of terminal lobe/length medium medium of blade shape ofapex of terminal lobe right-angled right-angled intensity of green colordark very dark blistering strong strong undulation of margin strongmoderate dentation of margin strong medium ovary: color of vestiturewhite white Fruit parthenocarpy absent absent length long long at ediblematurity: fruit length (cm) 19.5 18.7 diameter medium medium at ediblematurity: fruit diameter at  4.8  4.5 medial (cm) ratio length/diameterlarge large core diameter in relation to diameter medium small of fruitshape in transverse section round round to angular shape of stem endobtuse obtuse shape of calyx end rounded rounded at edible maturity:fruit gram weight 277.9  234.6  skin color/mottling mottled or speckledmottled or speckled with yellow with yellow at edible maturity:yellowish extended less than ⅓ extended less than ⅓ blossom end stripesof the fruit length of the fruit length at edible maturity: predominantdark green dark green color at stem end at edible maturity: Predominant139A 137A color at stem end (RHS Color Chart value) at edible maturity:predominant light green light green color at blossom end at ediblematurity: predominant 137C 144D color at blossom end (RHS Color Chartvalue) at edible maturity: fruit neck shape not necked not necked atedible maturity: fruit tapering ends blunt or rounded ends blunt orrounded at edible maturity: stem end cross circular circular section atedible maturity: medial cross circular triangular section at ediblematurity: blossom end circular circular cross section ground color ofskin at market stage green green intensity of ground color of skinmedium medium at edible maturity: skin thickness thick thin at ediblematurity: skin ribs medium weak sutures present present creasing absentabsent at edible maturity: skin toughness tough tough at ediblematurity: skin luster dull dull at edible maturity: spine color whitewhite at edible maturity: spine quality fine fine at edible maturity:spine density few few type of vestiture prickles only prickles onlydensity of vestiture sparse very sparse density of vestiture (onlyvarieties white white with white ovary vestiture) warts absent absent atedible maturity: flavor bitterfree bitterfree length of stripes shortabsent or very short dots present absent distribution of dots evenlydistributed N/A length of fruit containing dots whole length N/A densityof dots sparse N/A glaucosity medium absent or very weak length ofpeduncle long long ground color of skin at physiological green yellowripeness Fruit seed at harvest maturity measurements fruit seed length(cm)  0.9  0.80 measurements fruit seed diameter at  0.32  0.31 medial(cm) color cream cream color RHS Color Chart value 158A 161D colorpattern not striped not striped surface smooth smooth netting slight ornone slight or none Seeds number of seeds per fruit 29.5 53.7 grams per1,000 seeds 32.0 32.1These are typical values. Values may vary due to environment. Valuesthat are substantially equivalent are within the scope of the invention.

C. Breeding Cucumber Plants

One aspect of the current invention concerns methods for producing seedof cucumber hybrid SV8592CS involving crossing cucumber lineASL-147-1002-GY and cucumber line ASL-M3092029MO. Alternatively, inother embodiments of the invention, cucumber hybrid SV8592CS may becrossed with itself or with any second plant. Such methods can be usedfor propagation of cucumber hybrid SV8592CS or can be used to produceplants that are derived from cucumber hybrid SV8592CS. Plants derivedfrom cucumber hybrid SV8592CS may be used, in certain embodiments, forthe development of new cucumber varieties.

The development of new varieties using one or more starting varieties iswell-known in the art. In accordance with the invention, novel varietiesmay be created by crossing cucumber hybrid SV8592CS followed by multiplegenerations of breeding according to such well-known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g., colchicine treatment). Alternatively, haploid embryos may begrown into haploid plants and treated to induce chromosome doubling. Ineither case, fertile homozygous plants are obtained. In accordance withthe invention, any of such techniques may be used in connection with aplant of the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withcucumber hybrid SV8592CS for the purpose of developing novel cucumberlines, it will typically be preferred to choose those plants whicheither themselves exhibit one or more selected desirable characteristicsor which exhibit the desired characteristic(s) when in hybridcombination. Examples of desirable traits may include, in specificembodiments, high seed yield, high seed germination, seedling vigor,high fruit yield, disease tolerance or resistance, and adaptability forsoil and climate conditions. Consumer-driven traits, such as a fruitshape, color, texture, and taste are other examples of traits that maybe incorporated into new lines of cucumber plants developed by thisinvention.

D. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those cucumber plants which are developed by aplant breeding technique called backcrossing or by genetic engineering,wherein essentially all of the morphological and physiologicalcharacteristics of a variety are recovered or conserved in addition tothe single locus introduced into the variety via the backcrossing orgenetic engineering technique, respectively. By essentially all of themorphological and physiological characteristics, it is meant that thecharacteristics of a plant are recovered or conserved that are otherwisepresent when compared in the same environment, other than an occasionalvariant trait that might arise during backcrossing, introduction of atransgene, or application of a genetic engineering technique.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalcucumber plant which contributes the locus for the desiredcharacteristic is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental cucumber plant to which the locus or loci from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a cucumber plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny cucumber plants of a backcross in which aplant described herein is the recurrent parent comprise (i) the desiredtrait from the non-recurrent parent and (ii) all of the physiologicaland morphological characteristics of cucumber the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiol., 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of cucumber plants for breeding is not necessarily dependenton the phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming, or otherwise disadvantageous. In addition,marker assisted selection may be used to identify plants comprisingdesirable genotypes at the seed, seedling, or plant stage, to identifyor assess the purity of a cultivar, to catalog the genetic diversity ofa germplasm collection, and to monitor specific alleles or haplotypeswithin an established cultivar.

Types of genetic markers which could be used in accordance with theinvention include, but are not necessarily limited to, Simple SequenceLength Polymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In particular embodiments of the invention, marker assisted selection isused to increase the efficiency of a backcrossing breeding scheme forproducing a cucumber line comprising a desired trait. This technique iscommonly referred to as marker assisted backcrossing (MABC). Thistechnique is well-known in the art and may involve, for example, the useof three or more levels of selection, including foreground selection toidentity the presence of a desired locus, which may complement orreplace phenotype screening protocols; recombinant selection to minimizelinkage drag; and background selection to maximize recurrent parentgenome recovery.

E. Plants Derived by Genetic Engineering

Various genetic engineering technologies have been developed and may beused by those of skill in the art to introduce traits in plants. Incertain aspects of the claimed invention, traits are introduced intocucumber plants via altering or introducing a single genetic locus ortransgene into the genome of a recited variety or progenitor thereof.Methods of genetic engineering to modify, delete, or insert genes andpolynucleotides into the genomic DNA of plants are well-known in theart.

In specific embodiments of the invention, improved cucumber lines can becreated through the site-specific modification of a plant genome.Methods of genetic engineering include, for example, utilizingsequence-specific nucleases such as zinc-finger nucleases (see, forexample, U.S. Pat. Appl. Pub. No. 2011-0203012); engineered or nativemeganucleases; TALE-endonucleases (see, for example, U.S. Pat. Nos.8,586,363 and 9,181,535); and RNA-guided endonucleases, such as those ofthe CRISPR/Cas systems (see, for example, U.S. Pat. Nos. 8,697,359 and8,771,945 and U.S. Pat. Appl. Pub. No. 2014-0068797). One embodiment ofthe invention thus relates to utilizing a nuclease or any associatedprotein to carry out genome modification. This nuclease could beprovided heterologously within donor template DNA for templated-genomicediting or in a separate molecule or vector. A recombinant DNA constructmay also comprise a sequence encoding one or more guide RNAs to directthe nuclease to the site within the plant genome to be modified. Furthermethods for altering or introducing a single genetic locus include, forexample, utilizing single-stranded oligonucleotides to introduce basepair modifications in a cucumber plant genome (see, for example Sauer etal., Plant Physiol, 170(4):1917-1928, 2016).

Methods for site-directed alteration or introduction of a single geneticlocus are well-known in the art and include those that utilizesequence-specific nucleases, such as the aforementioned, or complexes ofproteins and guide-RNA that cut genomic DNA to produce a double-strandbreak (DSB) or nick at a genetic locus. As is well-understood in theart, during the process of repairing the DSB or nick introduced by thenuclease enzyme, a donor template, transgene, or expression cassettepolynucleotide may become integrated into the genome at the site of theDSB or nick. The presence of homology arms in the DNA to be integratedmay promote the adoption and targeting of the insertion sequence intothe plant genome during the repair process through homologousrecombination or non-homologous end joining (NHEJ).

In another embodiment of the invention, genetic transformation may beused to insert a selected transgene into a plant of the invention ormay, alternatively, be used for the preparation of transgenes which canbe introduced by backcrossing. Methods for the transformation of plantsthat are well-known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation, anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Nat. Biotechnol., 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Nat. Biotechnol., 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, for example,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13:344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, for example, Odel et al., Nature, 313:810,1985), including in monocots (see, for example, Dekeyser et al., PlantCell, 2:591, 1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389,1990); a tandemly duplicated version of the CaMV 35S promoter, theenhanced 35S promoter (P-e35S); the nopaline synthase promoter (An etal., Plant Physiol., 88:547, 1988); the octopine synthase promoter(Fromm et al., Plant Cell, 1:977, 1989); the figwort mosaic virus(P-FMV) promoter as described in U.S. Pat. No. 5,378,619; an enhancedversion of the FMV promoter (P-eFMV) where the promoter sequence ofP-FMV is duplicated in tandem; the cauliflower mosaic virus 19Spromoter; a sugarcane bacilliform virus promoter; a commelina yellowmottle virus promoter; and other plant virus promoters known to expressin plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, or developmental signals can also beused for expression of an operably linked gene in plant cells, includingpromoters regulated by (1) heat (Callis et al., Plant Physiol., 88:965,1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., PlantCell, 1:471, 1989; maize rbcS promoter, Schaffner and Sheen, Plant Cell,3:997, 1991; or chlorophyll a/b-binding protein promoter, Simpson etal., EMBO J., 4:2723, 1985), (3) hormones, such as abscisic acid(Marcotte et al., Plant Cell, 1:969, 1989), (4) wounding (e.g., wunl,Siebertz et al., Plant Cell, 1:961, 1989); or (5) chemicals, such asmethyl jasmonate, salicylic acid, or Safener. It may also beadvantageous to employ organ-specific promoters (e.g., Roshal et al.,EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a cucumber plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a cucumber plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see, for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

F. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a genetic locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions or transgenes from one geneticbackground into another.

Crossing: The mating of two parent plants.

Cross-Pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence, or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) Color Chart Value: The RHS Color Chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightness,and saturation. A color is precisely named by the RHS Color Chart byidentifying the group name, sheet number, and letter, e.g.,Yellow-Orange Group 19A or Red Group 41B.

Self-Pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing or genetic engineering ofa locus, wherein essentially all of the morphological and physiologicalcharacteristics of a cucumber variety are recovered in addition to thecharacteristics of the single locus.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a cucumber plant by transformation orsite-specific modification.

G. Deposit Information

A deposit of cucumber hybrid SV8592CS, disclosed above and recited inthe claims, has been made with the American Type Culture Collection(ATCC), 10801 University Blvd., Manassas, Va. 20110-2209. The date ofdeposit for cucumber hybrid SV8592CS is Dec. 10, 2014. The accessionnumber for those deposited seeds of cucumber hybrid SV8592CS is ATCCAccession Number PTA-121834. Upon issuance of a patent, all restrictionsupon the deposit will be removed, and the deposit is intended to meetall of the requirements of 37 C.F.R. §§ 1.801-1.809. The deposit will bemaintained in the depository for a period of 30 years, 5 years after thelast request, or the effective life of the patent, whichever is longer,and will be replaced if necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

1. A cucumber plant of cucumber hybrid SV8592CS, a sample of seed ofsaid hybrid having been deposited under ATCC Accession NumberPTA-121834.
 2. A cucumber seed that produces the plant of claim
 1. 3. Aplant part of the plant of claim 1, wherein the plant part comprises acell of said plant.
 4. A cucumber plant having all the physiological andmorphological characteristics of the plant of claim
 1. 5. A tissueculture of regenerable cells of the plant of claim
 1. 6. A cucumberplant regenerated from the tissue culture of claim 5, wherein said planthas all of the morphological and physiological characteristics of saidcucumber hybrid SV8592CS.
 7. A method of vegetatively propagating thecucumber plant of claim 1, the method comprising the steps of: (a)collecting tissue capable of being propagated from the plant of claim 1;and (b) propagating a cucumber plant from said tissue.
 8. A method ofproducing a cucumber plant comprising an added trait, the methodcomprising introducing a transgene conferring the trait into the plantof claim
 1. 9. A cucumber plant produced by the method of claim 8,wherein said plant comprises the trait and otherwise comprises all ofthe physiological and morphological characteristics of cucumber hybridSV8592CS.
 10. A cucumber plant of cucumber hybrid SV8592CS, a sample ofseed of said hybrid having been deposited under ATCC Accession NumberPTA-121834, further comprising a transgene, wherein said plant comprisesthe transgene and otherwise comprises all of the physiological andmorphological characteristics of cucumber hybrid SV8592CS.
 11. The plantof claim 10, wherein the transgene confers a trait selected from thegroup consisting of male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, modified fatty acidmetabolism, environmental stress tolerance, modified carbohydratemetabolism, and modified protein metabolism.
 12. A cucumber plant ofcucumber hybrid SV8592CS, a sample of seed of said hybrid having beendeposited under ATCC Accession Number PTA-121834, further comprising asingle locus conversion, wherein said plant comprises the single locusconversion and otherwise comprises all of the physiological andmorphological characteristics of cucumber hybrid SV8592CS.
 13. The plantof claim 12, wherein the single locus conversion confers a traitselected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism, and modified protein metabolism.
 14. A methodfor producing a seed of a cucumber plant derived from cucumber hybridSV8592CS, the method comprising the steps of: (a) crossing the cucumberplant of claim 1 with itself or a second cucumber plant; and (b)allowing seed of a hybrid SV8592CS-derived cucumber plant to form.
 15. Amethod of producing a seed of a hybrid SV8592CS-derived cucumber plant,the method comprising the steps of: (a) producing a hybridSV8592CS-derived cucumber plant from a seed produced by crossing thecucumber plant of claim 1 with itself or a second cucumber plant; and(b) crossing the hybrid SV8592CS-derived cucumber plant with itself or adifferent cucumber plant to obtain a seed of a further hybridSV8592CS-derived cucumber plant.
 16. The method of claim 15, the methodfurther comprising repeating said producing and crossing steps of (a)and (b) using the seed from said step (b) for producing a plantaccording to step (a) for at least one generation to produce a seed ofan additional hybrid SV8592CS-derived cucumber plant.
 17. A method ofproducing a cucumber fruit, the method comprising: (a) obtaining theplant of claim 1, wherein the plant has been cultivated to maturity; and(b) collecting a cucumber fruit from the plant.